Virology Reagents

The main vectors of gene therapy in research are viruses. The most popular tool for gene delivery is a genetically modified lentivirus. Modified lentivirus (HIV-1) vectors retain their ability to infect undivided cells, thereby increasing their ability to transduce a wide variety of cells, including those that are difficult to transduce. This advantage enables the stable long-term expression of a transgene. In immunotherapy, CAR-T cells are manufactured by transducing the CAR gene with an HIV-1 vector in T cells to express a specific chimeric p24 protein on their surface. This allows them to recognize cancer cells and destroy them. These CAR-T cells must be generated individually to treat each patient. This application note demonstrates a comparative quantification of the p24 titer in a lentiviral GFP control sample using Alliance HIV-1 p24 Antigen ELISA and p24 AlphaLISA immunoassay platforms. Check out the different sections of this application note: A lentiviral vector that encodes the CAR construct Determination of the efficiency of transient co-transfection by measuring viral titer Detection of the presence of a p24 HIV-1 specific antibody in the sample Quantification of targets present in the sample

Discover Alpha SureFire ®   Ultra ™ assays, the no-wash cellular kinase assays leveraging Revvity's exclusive bead-based technology and sandwich immunoassays for detecting phosphorylated proteins in cells. Offering a quantitative alternative to Western Blotting, Alpha SureFire assays are automation-friendly, easily miniaturized, and proficient in detecting both endogenous and recombinant proteins. Explore our comprehensive portfolio of SureFire Assays, designed to help you elevate and expedite your drug discovery journey.

This guide outlines further possible optimization of cellular and immunoassay parameters to ensure the best possible results are obtained.

This application note explores how the AlphaLISA™ SureFire® Ultra™ technology reveals the intricacies of TREM2/DAP12 signaling in neuroinflammation.

The definitive guide for setting up a successful AlphaLISA SureFire Ultra assay Several biological processes are regulated by protein phosphorylation. It is, therefore, no surprise that the dysregulation of protein phosphorylation is implicated in a relatively large number of diseases. AlphaLISA SureFire Ultra assays provide a robust and reliable method for quantifying a targeted phosphorylation event in cell-based experiments. This guide contains tools and data helpful for you to perform your assays using AlphaLISA SureFire Ultra: A detailed description of the assay and its options A thorough investigation of assay conditions to obtain the optimal response from the chosen modulator and cell line A list of optimization steps to provide a sufficient assay window and produce the strongest results possible

Atherosclerosis pathogenesis, cellular actors, and pathways Atherosclerosis is a common condition in which arteries harden and become narrow due to a build-up of fatty material, usually cholesterol, and other substances such as calcium. This can lead to a range of serious health complications, including heart attack or stroke, making the disease an important contributing factor in death and morbidity in developed countries. Recent developments in our understanding of atherosclerosis from a molecular perspective include the discovery of new players in disease pathogenesis. Included in this white paper Atherosclerosis: step-by-step pathogenesis, therapeutic strategies, and recent developments Detailed descriptions and explanations, including a focus on pathways

With over 200 different types of cancer, management relies on a variety of techniques such as chemotherapy, radiotherapy, and surgery. These types of therapies can be associated with severe side effects, and finding safer and more effective treatments is a priority in cancer therapeutics research. One approach that has shown huge potential is monoclonal antibody-based cancer therapeutics. In this white paper we explore this exciting area of anti-cancer research, covering mechanism of action, development, and challenges in monoclonal antibody-based therapeutics, including antibody-drug conjugates and multispecific antibodies.

In this application note three ATP monitoring luminescence assays were assessed and compared head-to-head: (i) ATPlite™ from Revvity, (ii) ATPlite™ 1step from Revvity and (iii) ATP assay kit from a competitor company.

Immunoassays are a mainstay for the quantification of a variety of biomolecular analytes in drug discovery, drug development, and life sciences research. AlphaLISA® proves advantageous to ELISAs, offering a novel, homogenous immunoassay technology that eliminates wash steps. Using the JANUS® Automated Workstation in combination with AlphaLISA provides a solution to easily preparing assays that can be tailored to the needs of the laboratory. Moreover, the JANUS workstation can be easily set-up to process different assay types in a multi-user, multi-assay environment, thus providing flexible automated workflows.

Taking autophagy regulation research a step further Autophagy regulation is a key molecular process involved in recycling long-lived protein and organelles. Dysregulation of autophagy leads to different pathologies such as cancer, neurodegenerative and infectious diseases. This eBook features: Key facts about autophagy and mitophagy Infographics to apprehend the basics Cutting-edge knowledge

AlphaLISA™ technology is a highly sensitive, easy-to-use, and reproducible method for detecting and quantifying molecules in various biological matrices. It works by using streptavidin-coated Donor beads and biotinylated anti-analyte antibodies. When these come into close proximity, the excitation of the Donor beads at 680 nM triggers an energy transfer cascade in the Acceptor beads, generating a sharp emission peak at 615 nM. However, some cell culture media contain high levels of biotin, which can interfere with AlphaLISA and other assay technologies that rely on a streptavidin-biotin binding event for detection. High levels of free biotin in the sample matrix can result in a decrease in total counts, lower signal to background ratios, and reduced AlphaLISA assay detection limits. To mitigate this, AlphaLISA biotin-free kits have been developed. This application note demonstrates the value of using AlphaLISA biotin-free kits to reduce the effects of biotin interference in sample and standard preparations.

Get a useful overview of today’s NAFLD knowledge with this booklet. NASH disease is complex and follows many development pathways. This booklet provides you with critical information regarding NAFLD and more specifically about NASH progression. Review the fundamentals of NAFLD and NASH learn from an important research report Benefit from additional content to help your NASH research

Get a useful overview of today’s immunity knowledge with this booklet Immunity is a collection of complex processes involving multiple strategies and specialized cell types. This booklet provides you with critical information regarding their roles, characteristic and signaling pathways as well as the collaborative behaviors that contribute to immunity. Featured in this guide: Review the fundamentals of immune cell types and mechanisms Learn from a cutting-edge research report Pathways and functional details on over 10 specialized immune cells

Cytokine Detection Using AlphaLISA Biotin-Free assays This technical note describes the use of AlphaLISA biotin-free kits for detecting and quantifying cytokines, including interleukin 2 (IL-2), tumor necrosis factor alpha (TNFα), interferon gamma (IFN-γ), and interleukin 6 (IL-6), in supernatants produced by human PBMCs cultured for two days with or without Dynabead ® stimulation.

The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both antibodies and small molecules. CCR7 and CXCR2 receptors, which are expressed in immune cells and are therapeutic targets for disorders like lupus erythematosus, adult leukemia, lymphomas, chronic obstructive pulmonary disease (COPD), and sepsis. AlphaLISA ™ SureFire ® Ultra ™ and Alpha SureFire ® Ultra ™ Multiplex assays are automation-friendly, applicable to both small and large-scale screens, and can assess phosphorylation status in complex matrices. The LANCE Ultra cAMP assay is measures cyclic AMP (cAMP) produced upon modulation of adenylyl cyclase activity by G-protein coupled receptors (GPCRs). This application note demonstrates how the SureFire Ultra and LANCE Ultra cAMP assays can be used for measuring inhibitors to CCR7 and CXCR2 cell surface receptors using a cellular model system where these receptors are overexpressed in CHO cells. The assays were optimized to measure receptor blockage and assayed receptor activity modulation by detecting ERK and AKT phosphorylation status and cAMP modulation. For more details, download the application note!

AlphaLISA and LANCE (TR-FRET) biomarker assays can be used to measure ECM-associated protein modulation caused by human transforming growth factor-beta (TGF-β) induction of EMT in a 3D Spheroid model of human prostate carcinoma.

The definitive guide to setting up a successful cytokine assay Many therapeutic areas require an understanding of cytokine release. When preparing for a cytokine assay, many underappreciated parameters (e.g. sample handling, cell culture format, sample dilutions) can in fact greatly impact the performance of the cytokine detection. This guide reviews the latest knowledge surrounding the proper use of HTRF cytokine assays. A review of the key terms and definitions in cytokine detection A list of optimization steps Recommendations for data analysis

Ask real blood for real responses Fresh blood is the model of choice to study drug immunotoxicity and predict adverse effects. Written in collaboration with Blood Assay Solutions, this note provides guidelines for fresh blood cytokine quantification. Discover the power of our cytokine portfolio for your research. Features Step by step protocols for fresh blood cytokine quantification in your research Examples of pathway stimulation assays (TCR, TLR …) Comparison beteen fresh blood and PBMCs

Cytokines are implicated in the pathology and outcome of disease states across all therapeutic areas. When preparing to run cytokine assays, it is key to consider a multitude of factors that impact the assay performance, such as sample dilutions and cell culture format. Utilize this guide to optimize your AlphaLISA ™ cytokine assays with in-depth data and information!

Discover a rapid and reliable tool for the identification and characterization of STING-targeting compounds. Quantifying human IFN-β using an HTRF-based approach This note demonstrates how using HTRF to quantify human IFN-β is a powerful and effective method for the characterization of STING agonists. It provides data demonstrating that the quantification of IFN-β using HTRF is well correlated with ELISA assays. This note also provides data about gene reporter assay correlation with our HTRF IFN-β assay.

Cytotoxicity, cell viability, and cell proliferation are important parameters to monitor when developing new therapeutics. For almost 20 years, Revvity has offered two options for the measurement of cellular levels of Adenosine Tri-Phosphate (ATP) from mammalian cell monolayers, as a marker of cell viability and proliferation. Both the ATPlite™ and ATPlite 1step assays (2D and 3D) are based on the production of light caused by the reaction of cellular ATP with added firefly luciferase and D-luciferin. Check out our flyer to have our latest solutions for Cytotoxicity, cell viability, and cell proliferation assays. For research use only. Not for use in diagnostic procedures.

Although every cancer patient has the ability to respond to immunotherapy, tumors often fail to regress due to either primary or acquired resistance. In our interview, Dr. Siwen Hu-Lieskovan, PhD, MD, Director of Solid Tumor Immunotherapy at the Huntsman Cancer Institute, University of Utah discussed the mechanisms behind immune evasion and how this understanding can help create a personalized treatment strategy with the goal of prolonged survival.

Dive deeper into fibrosis research In fibrotic disorders, the normal regulation of the extracellular matrix (ECM) is compromised, which leads to excess of ECM or lack of MMPs. This can further lead to a disorganized accumulation of ECM which can ultimately result in organ rigidity and fibrosis. For this reason, the ECM constitutive proteins, MMPs and fibroblasts activating and regulatory mediators such as TGF-β1 and PGE2 are hot spots of therapeutic research for fibrosis. With this review, you will: Better understand how COX enzymes regulate PGE2 levels in TLR stimulated fibroblasts Learn how an AlphaLISA protein-protein interaction assay can be used as a screening platform for TG2-Fibronectin inhibitor identification Discover how CKD might impact the malignant myocardial fibrosis factor combination

Discover the cutting-edge of luminescence assay technology, offering unparalleled sensitivity and dynamic range for applications in gene expression, cell viability, and drug development. Experience high-throughput, cost-effective detection with minimal interference and simple automation. Check out our brochure to learn more about how this innovative technology can enhance your research studies. For research use only. Not for use in diagnostic procedures.

A comprehensive overview of fibrosis development Fibrosis is a main contributor to a wide range of organ failures which stems from a mis-regulation of inflammation in response to injury. Fibrosis occurs when the long-term remodeling and removal of ECM suffers from a higher production than removal rate. This event is associated with chronic inflammation, which keeps myofibroblasts active for extended periods, thus making them and their ECM protein secretion the main effectors of pathological fibrosis. This guide is intended to help scientists and researchers appreciate and navigate the cellular and signaling actors of the development of fibrotic pathologies and disorders. Review the fundamentals of fibrosis as a process. A look at TGF-β1 signaling pathways as therapeutic approaches for fibrotic disorders. Insight into immune cell types involved and treating and reversing progressive.