The LANCE® Ultra Mouse IL-6 Detection Kit is designed for detection and quantitation of murine IL-6 in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Feature | Specification |
---|---|
Application | Protein Quantification |
Dynamic Range | 8.2- 30,000 pg/mL |
Limit of Detection | 8.2 pg/mL |
Limit of Quantification | 42.5 pg/mL |
Sample Volume | 15 µL |
The LANCE® Ultra Mouse IL-6 Detection Kit is designed for detection and quantitation of murine IL-6 in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
The LANCE Ultra Mouse IL-6 Detection Kit is designed for detection and quantitation of murine IL-6 in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
The 500 point kit contains enough reagents to run 500 wells in 384-well format, using a 20 µL reaction volume (15 µL of sample). The 10,000 point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 µL reaction volume (15 µL of sample).
LANCE and LANCE (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.
In the mouse, Interleukin 6 (IL6) is a 187 amino acid cytokine. In humans, IL6 is produced by a variety of cell types, including T-cells, mast cells, and monocytes. IL6 signals through binding to the gp130/ IL6R receptor complex. IL6 acts not only on the immune system, but also affects many physiological events in various organs. IL6 exerts pro- or antiinflammatory responses, depending on the target cell analyzed and the in vivo environmental circumstances. IL6 is a differentiation and proliferation factor for B and T cells, and acts as a migration factor on monocytic cells. It is the major activator of acute-phase protein expression in the liver and acts as a survival factor on neuronal cells. Elevated serum IL6 levels have been observed during bacterial and viral infection, trauma, autoimmune disease, inflammation, and cancer.
Application |
Protein Quantification
|
---|---|
Automation Compatible |
Yes
|
Brand |
LANCE Ultra
|
Detection Modality |
LANCE
|
Dynamic Range |
8.2- 30,000 pg/mL
|
Limit of Detection |
8.2 pg/mL
|
Limit of Quantification |
42.5 pg/mL
|
Product Group |
Kit
|
Sample Volume |
15 µL
|
Shipping Conditions |
Shipped in Blue Ice
|
Target |
IL6
|
Target Class |
Cytokines
|
Target Species |
Mouse
|
Technology |
TR-FRET
|
Therapeutic Area |
Inflammation
|
Unit Size |
10,000 assay points
|
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