Revvity Sites Globally
Select your location.
*e-commerce not available for this region.
HTRF Prostaglandin E2 Detection Kit, 10,000 Assay Points
HTRF Prostaglandin E2 Detection Kit, 10,000 Assay Points
HTRF Prostaglandin E2 Detection Kit
| Feature | Specification |
|---|---|
| Application | Protein Quantification |
| Dynamic Range | 8 - 1,000 pg/mL |
| Limit of Detection | 10 pg/mL |
| Sample Volume | 10 µL |
Product variants
Unit Size: 500 assay points
Part #:
62P2APEG
List price
USD 1,131.61
Your online price:
Unit Size: 10,000 assay points
Part #:
62P2APEH
List price
USD 12,243.50
Your online price:
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).
HTRF Prostaglandin E2 Detection Kit, 10,000 Assay Points
HTRF Prostaglandin E2 Detection Kit
HTRF Prostaglandin E2 Detection Kit, 10,000 Assay Points
Product information
Overview
Prostaglandin E2 (PGE2) is produced in multiple cell types from PGH2, a primary product of the arachidonic acid metabolism, via the prostaglandin synthase. PGE2 displays several biological functions, such as vasodilatation and smooth muscle relaxation, and is involved in pro- and anti-inflammation pathways. PGE2 production is a commonly used method for the detection of COX-1 and COX-2 modulation. The PGE2 kit is a highly sensitive method for quantifying PGE2, either in cell supernatant or directly in whole cells.
How it works
Assay principle
The PGE2 assay is based on the competition principle, where native PG2 produced by cells and d2-labelled PGE2 compete for binding to a monoclonal anti-PGE2 antibody labelled with Europium Cryptate. The assay can be run with several types of samples, such as cell supernatants or purified enzymes.
Assay Protocol
The PGE2 assay features a streamlined protocol with only 1 incubation step after sample and PGE2 detection reagents dispensing. This protocol requires a single 5-hour incubation period at RT.
Assay details
Key features
The PGE2 assay has a high level of flexibility. It can be performed using cell supernatants or directly on stimulated cells. The incubation time and temperature following addition of the detection reagents have little effect on the assay results, providing further assay flexibility.
| Detection limit | 10 pg/mL |
|---|---|
| Dynamic range | 8 to 1000 pg/mL |
| EC50 | 250 pg/mL |
| S/B | 31 |
| Z' | 0.9 for 20 µl assay volumes |
Assay validation
PGE2 modulation upon Indomethacin inhibitor treatment
One benefit of the HTRF PGE2 assay is its ability to quantify PGE2 in the presence of cells without sacrificing assay performance. The results comparing direct cell-based versus supernatant transfer did not differ significantly. Monocytes were stimulated to produce PGE2 in the presence or absence of indomethacin, a known inhibitor of PGE2 production. The IC50 of indomethacin determined using HTRF technology (1.0 ± 0.4 nM) was in agreement with previously published data.
Specifications
| Application |
Protein Quantification
|
|---|---|
| Brand |
HTRF
|
| Detection Modality |
HTRF
|
| Dynamic Range |
8 - 1,000 pg/mL
|
| Limit of Detection |
10 pg/mL
|
| Product Group |
Kit
|
| Sample Volume |
10 µL
|
| Shipping Conditions |
Shipped Ambient
|
| Target Class |
Biomarkers
|
| Technology |
TR-FRET
|
| Therapeutic Area |
Cardiovascular
Neuroscience
Oncology & Inflammation
|
| Unit Size |
10,000 assay points
|
Video gallery
HTRF Prostaglandin E2 Detection Kit, 10,000 Assay Points
HTRF Prostaglandin E2 Detection Kit, 10,000 Assay Points
Resources
Are you looking for resources, click on the resource type to explore further.
Guide
HTRF solutions, guide to major applications
This guide provides you an overview of HTRF applications in several therapeutic areas.
Recently viewed
Part number:
CSK-A0007-1,
CSK-A0007-2
List price:
USD 121.16 - USD 165.22
Log in to view
your online price
How can we help you?
We are here to answer your questions.
