Cell Signaling Research Reagents

Get the best out of your phosphorylation assays Combining phospho and total protein assays enables better analysis. This Application Note provides valuable guidelines for efficiently analyzing and interpreting results from such assay combinations. Check out all the tips and examples in features! Features Introduction to phospho and total protein assay relevance Tips for handling and interpreting data Examples from actual experiments

Protein-protein interactions: cover them all with one approach This brochure illustrates the possibilities and versatility of protein-protein interaction studies. It features six relevant examples of various interaction types taken from literature to show you how studies can be handled with the time-resolved fret-based HTRF approach, including virus blockade, receptor/ligand binding, protease activity, and more. Features: Introduction to the stakes of protein-protein interaction research Illustration of 6 published interaction studies involving biologics or small molecules For research use only. Not for use in diagnostic procedures.

The RAS family of genes, particularly K-Ras, plays a critical role in cancer biology. Despite its notorious difficulty as a therapeutic target, recent breakthroughs have brought new hope in treating cancers driven by K-Ras mutations. Our latest application note delves into innovative approaches to K-Ras inhibition, including small molecule inhibitors, synthetic lethality strategies, and PROTAC® molecules. We also showcase the high specificity and sensitivity of the no-wash HTRF™ K- Ras immunoassay, a cutting-edge tool that offers a reliable and precise method for evaluating K-Ras protein levels, outperforming traditional techniques. Discover how this assay can accelerate your research in targeting the elusive K-Ras.

Discover Alpha SureFire ®   Ultra ™ assays, the no-wash cellular kinase assays leveraging Revvity's exclusive bead-based technology and sandwich immunoassays for detecting phosphorylated proteins in cells. Offering a quantitative alternative to Western Blotting, Alpha SureFire assays are automation-friendly, easily miniaturized, and proficient in detecting both endogenous and recombinant proteins. Explore our comprehensive portfolio of SureFire Assays, designed to help you elevate and expedite your drug discovery journey.

Quantifying H3K27ac Levels with AlphaLISA Assay In this technical note, you will discover how AlphaLISA immunodetection assay easily detects alterations in acetylated histone H3 lysine 27 (H3K27ac) levels within cellular extracts.

Quantifying H3K9ac in Cellular Extracts with AlphaLISA This technical note gives you details about how AlphaLISA immunodetection assay monitors changes in the levels of acetylated histone H3 lysine 9 (H3K9ac) in cellular extracts.

Quantifying Immune Checkpoints: AlphaLISA Biomarker Detection Kits This technical note focuses on the role of checkpoint molecules in cancer immunotherapies. Checkpoint molecules are molecular markers that can regulate an immune system attack on cancer cells. They have gained prominence as promising candidates for immunotherapies. AlphaLISA detection kits are designed to detect and quantify the levels of these molecules in cell culture media, serum, and cell lysates.

Decoding Histone Modifications: A Targeted Assay for H3K4me2 In this study, you will find details about an optimized assay designed to quantify changes in H3K4me2 levels following treatment with sodium butyrate and Trichostatin A (TSA), both of which are non-selective histone deacetylase (HDAC) inhibitors. Using a standardized histone extraction procedure, we detect the target mark by employing a biotinylated anti-Histone H3 (C-terminus) antibody and AlphaLISA Acceptor beads conjugated to an antibody specific to the mark.

Quantification of H3K27me3 Levels Using AlphaLISA Technology In this technical note discover how AlphaLISA ™ immunodetection assay tracks variations in cellular extract levels of di-methylated histone H3 lysine 27 (H3K27me2-1).

Unlocking the secrets of epigenetics, our technical not dives into optimizing a DOT1L enzymatic assay This technical document outlines the optimization of a DOT1L enzymatic assay using oligonucleosomes as the substrate. Discover how the assay easily detects the dimethylated product of histone H3 lysine 79 by leveraging a biotinylated anti-H3K79me2 antibody, which binds to the epigenetic mark of interest.

Lysine 27 Dimethylation Detection with AlphaLISA Technology In this technical note discover how AlphaLISA immunodetection assay detects dimethylation of a biotinylated Histone H3 (21-44) peptide at lysine 27. It uses anti-di/mono-methyl-Histone H3 Lysine 27 (H3K27me2-1) as the detection antibody.

AlphaLISA ™ Detects β2-Microglobulin in Urine with High Sensitivity When kidney tubular function is impaired, elevated levels of β2-microglobulin in urine indicate renal filtration or reabsorption disorders. Measuring urinary β2-microglobulin can help assess tubular function, but variability in urine composition may affect results. In this study, AlphaLISA ™ technology is demonstrated for the first time in the complex matrix of urine, and is shown to detect the renal tubular injury marker Beta2-microglobulin (β2-microglobulin), in urine at a sensitivity of 77 pg/mL.

Quantification of Histone H3 Lysine 9 Di-Methylation Using AlphaLISA Assay In this technical note discover how The AlphaLISA assay detects di-methylation of a biotinylated Histone H3 (1-21) peptide at lysine 9. In this assay, a biotinylated histone H3-derived peptide serves as the substrate, and the modified substrate is detected using Streptavidin (SA) Alpha Donor beads and AlphaLISA Acceptor beads conjugated to an antibody specific to the epigenetic mark of interest.

AlphaLISA Assay Insights: Tracking Deacetylation Dynamics on Histone H3 Peptides In this technical note discover how this AlphaLISA assay is designed to detect the removal of acetyl groups from a biotin-tagged peptide derived from Histone H3, specifically the segment from amino acids 21 to 44, which has been acetylated at the lysine 27 position. For research use only. Not for use in diagnostic procedures.

AlphaLISA ™ Technology: Unveiling KIM-1 Detection in Urinary Matrices In this technical note discover how AlphaLISA ™ technology is used in the complex matrix, urine. The variability of urine matrix components can affect antibody binding and assay performance. In this note, AlphaLISA technology was shown to detect the kidney injury marker, KIM-1, in urine down to 14 pg/mL. For research use only. Not for use in diagnostic procedures

Measuring Lysine 27 Tri-Methylation Demethylation in Histone H3 with AlphaLISA In this technical note, you will discover how this AlphaLISA immunodetection assay detects the demethylation of a biotinylated Histone H3 (21-44) peptide that is tri-methylated at lysine 27.

AlphaLISA KAT5 (TIP60) Assay for Detecting Histone H4 Acetylation In this technical note, you will discover how this AlphaLISA™ immunoassay detects the acetylation of the N-terminal lysine residues on a Histone H4 (1-25) peptide. For research use only. Not for use in diagnostic procedures.

AlphaLISA-Based Assay for LSD1 Activity on Histone H3-Lysine 4 In this technical note discover how this AlphaLISA immunoassay detects the demethylation of a biotinylated Histone H3 (1-21) peptide that is mono-methylated at lysine 4. For research use only. Not for use in diagnostic procedures.

AlphaLISA Assay for PRMT1-Mediated Methylation of Histone H4 at Arginine 3 In this technical note dsicover how this AlphaLISA™ immunoassay detects the methylation of a biotinylated histone H4 (1-21) peptide specifically at arginine 3.

Detection of PRMT4-Mediated Histone H3 Methylation at Arginine 26 using AlphaLISA In this technical note, discover how this AlphaLISA™ immunoassay detects the methylation of a biotin-labeled histone H3 peptide (amino acids 21-44) at arginine 26. For research use only. Not for use in diagnostic procedures.

Detection of Histone H3 Methylation Using AlphaLISA Assay In this technical note, you will discover how this AlphaLISA immunodetection assay detects the methylation of a biotinylated histone H3 (1-21) peptide at arginine 2.

AlphaLISA Assay: A Tool for Measuring Histone H3 Deacetylation In this technical note, you will discover how the AlphaLISA immunodetection assay is used to determine the deacetylation level of a Histone H3 peptide (from the 1st to the 21st amino acid) that has been biotinylated and acetylated at the 4th lysine residue.

This guide outlines further possible optimization of cellular and immunoassay parameters to ensure the best possible results are obtained.

This application note explores how the AlphaLISA™ SureFire® Ultra™ technology reveals the intricacies of TREM2/DAP12 signaling in neuroinflammation.

The definitive guide for setting up a successful AlphaLISA SureFire Ultra assay Several biological processes are regulated by protein phosphorylation. It is, therefore, no surprise that the dysregulation of protein phosphorylation is implicated in a relatively large number of diseases. AlphaLISA SureFire Ultra assays provide a robust and reliable method for quantifying a targeted phosphorylation event in cell-based experiments. This guide contains tools and data helpful for you to perform your assays using AlphaLISA SureFire Ultra: A detailed description of the assay and its options A thorough investigation of assay conditions to obtain the optimal response from the chosen modulator and cell line A list of optimization steps to provide a sufficient assay window and produce the strongest results possible