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Technical Note Icon   Technical Note
Comparison of cell concentration and viability using the Cellometer Auto 2000 cell counter and a manual hemocytometer.
Technical note comparing measurement of cell concentration and viability using the Cellometer Auto 2000 cell counter and a manual hemocytometer.
Technical Note Icon   Technical Note
Overcoming challenges in primary sample processing with animal infectious disease models using the Cellaca MX high-throughput cell counter.
Technical note describing how to address challenges in primary sample processing with animal infectious disease models using the Cellaca MX high-throughput cell counter.
Technical Note Icon   Technical Note
Biotin-free AlphaLISA assays for the detection of cytokines in PBMC supernatants
In this technical note, AlphaLISA biotin-free kits were used to detect and quantify the cytokines interleukin 2 (IL-2), tumor necrosis factor alpha (TNFα), interferon gamma (IFN-γ), and interleukin 6 (IL-6).
Technical Note Icon   Technical Note
Fast, simple, homogeneous assays for characterization of Fc receptor binding using AlphaLISA
In this technical note discover how the AlphaLISA human FcRn binding kit detects binding between FcRn (neonatal Fc receptor) and human IgG.
Technical Note Icon   Technical Note
Guidelines for cell culture and lysis in different formats prior to HTRF detection
This Technical Note will provide you with guidelines on adapting the standard 96-well plate protocol for seeding and lysing cells to different culture formats such as T75 flasks, or 6-well, 12-well, and 24-well plates.
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Technical Note NeoBase 2 on Sciex Triple Quad 4500MD - digital
Technical note NeoBase 2 on SCIEX Triple Quad 4500MD LC-MSMS System Digital.pdf
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Measure aggregated protein phosphorylation
This technical note shows that by combining the protein disaggregation kit with HTRF™ phospho immunoassays, you have a new, easy-to-use, and reliable approach for detecting all phosphorylation events during protein aggregation.
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AlphaLISA G9a Histone H3-Lysine N-methyltransferase assay
In this technical note discover how The AlphaLISA assay detects di-methylation of a biotinylated Histone H3 (1-21) peptide at lysine 9.
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AlphaLISA EZH2 Histone H3-Lysine 27 N-methyltransferase assay
AlphaLISA assay detects dimethylation of a biotinylated Histone H3 (21-44) peptide at lysine 27. It uses anti-di/mono-methyl-Histone H3 Lysine 27 (H3K27me2-1) as the detection antibody
Technical Note Icon   Technical Note
AlphaLISA SIRT1 Histone H3-Lysine 4 deacetylase assay
This AlphaLISA immunodetection assay measures the deacetylation of a biotinylated Histone H3 (1-21) peptide acetylated at lysine 4.
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A simple method for preparing GPCR membrane model extracts from stable cell lines for use with the HTRF GTP Gi binding assay
This Technical Note provides a detailed method for preparing GPCR membrane model extracts from stable cell lines for use with the HTRF GTP Gi binding assay, including cell culture preparation, cell lysis, membrane extraction, and assay optimization.
Technical Note Icon   Technical Note
AlphaLISA in urine: Detecting kidney injury marker KIM-1
This document highlights the capability of AlphaLISA technology to identify the kidney injury biomarker KIM-1 in urine samples, achieving detection levels as low as 14 pg/mL.
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