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Technical Note
Guidelines from PBMC isolation to cytokine assay optimization
This tech note will provide you with a complete step by step methodology to purify PBMCs and use them in cytokine quantification assays with HTRF
Technical Note
Guidelines for optimizing protein-protein interaction assays using HTRF PPI reagents
This technical note reviews the general principles and steps in developing a protein-protein interaction assay using HTRF PPi reagents from Revvity assays.
Technical Note
Guideline for PBMC freezing
This technical note describes a protocol for freezing and thawing PBMC and provides details on functional validations after freezing the procedure compared to fresh PBMC.
Technical Note
The Inositol-Phosphate approach, a robust and stable platform for HTS of Gq coupled GPCR
This technical note presents data showing the stability and ease-of-use of the assay, optimal choice for robotic high throughput screening campaigns.
Technical Note
Optimize your HTRF cell signaling assays for tissues
This technical note gives precise guidelines for tissue lysate preparation, assay optimization, data analysis, and then interpretation, guaranteeing you will get accurate and interpretable results.
Technical Note
Optimization and pharmacological validation of wild type beta-arrestin 2 plasmid transfection
This technical note provides details on how transfecting cell lines with a beta-arrestin plasmid can lead to an increase in beta-arrestin 2 recruitment signals and restoration of beta-arrestin 2 in otherwise deficient cells.
Technical Note
Smart Tray accessories for higher throughput optical imaging on the IVIS Spectrum 2 and IVIS SpectrumCT 2
Using Smart Tray accessories for higher throughput optical imaging on the IVIS Spectrum 2 and IVIS SpectrumCT 2.
Technical Note
Key success tips to perform β-arrestin 2 recruitment assays by using the B-arr2 recruitment kit with GPCRs
This technical note provides valuable tips for performing B-arr2 recruitment assays on adherent cell lines overexpressing GPCRs.
Technical Note
Lysis buffer compatibility HTRF assays
This leaflet presents the lysis buffer compatibility for HTRF assays.
Technical Note
TRF assay performance of the EnVision Nexus multimode plate reader
This technical note highlights the capabilities of the EnVision Nexus™ in delivering efficient results for HTRFTM and LANCETM immunoassays, establishing it as a leading system in the next generation of plate readers.
Technical Note
Guidelines for cell culture and lysis in different formats prior to HTRF detection
This Technical Note will provide you with guidelines on adapting the standard 96-well plate protocol for seeding and lysing cells to different culture formats such as T75 flasks, or 6-well, 12-well, and 24-well plates.
Technical Note
HTRF setup recommendations for EnVision
This document outlines setup options for properly configuring the EnVision multimode plate reader to achieve optimal HTRFTM readout.
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