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Application Note
HMGB1, IL-1β and IL-18 assays used together to discriminate pyroptosis and necroptosis
This application note shows that the release of HMGB1 induced in the context of pyroptosis and necroptosis can be easily monitored with the HTRF® HMGB1 kit.
Application Note
Download your application note on fibrosis characterization
This note describes deep investigations of the molecular mechanisms involved in tissue fibrosis, and occurring in the major effector cell types.
Application Note
Download your application note on nuclear receptor ligand identification with HTRF
This application note illustrates the use of HTRF PPI reagents to easily build robust and convenient biochemical NR/ CoA recruitment assays.
Application Note
Recommended Procedure For Binding Biotinylated Proteins to GrowDex®-A Jenni
Application note for GrowDex-A hydrogels.
Application Note
GrowDex-A Functionalized with Biotinylated Fibronectin and Vitronectin Promotes 3D Culture of Fibroblasts
Application note for GrowDex-A hydrogels.
Application Note
Kinetic Binding of Kinase inhibitors and determination of Kon, Koff rates
This application note presents the use of fluorescent kinase inhibitors to determine the kinetic binding properties of inhibitors of interest and demonstrates the pharmacological relevance of the kinase binding platform for both equilibrium-and kinetic profiling.
Application Note
HTRF analysis and imaging of phospho-S65-ubiquitin in cellular models of mitophagy
Discover how to utilize a no-wash Phospho Ubiquitin Serine 65 immunoassay specifically for studying mitophagy activation.
Application Note
Download your application note on STING agonists characterization with IFN-β
Discover a rapid and reliable tool for the identification and characterization of STING-targeting compounds.
Application Note
Download your application note on TGF-β SMAD α-SMA signaling in NASH
This application note illustrates the use of HTRF assays to analyze the status of the key molecular players involved in this signaling pathway.
Application Note
Characterizing pharmacological compounds with complementary Upstream and Downstream GPCR readouts
This application note presents and compares the pharmacological characterization of a panel of reference compounds (full agonists, partial agonists, antagonists) in δ-opioid and D2 receptor models with two different Gi-coupled receptor activity monitoring assays: the HTRF® GTP Gi binding assay and the HTRF cAMP Gi assay.
Application Note
FOXO1 in Non-Alcoholic Fatty Liver Disease
This application note demonstrates the applicability of the HTRF Phospho-FoxO1 (Ser256) and Total FoxO1 assays to study FoxO1 in HepG2 hepatocytes
Application Note
Research on neutralizing antibodies in autoimmune diseases
The data presented here demonstrates that HTRF biochemical and cellular assays offer a comprehensive and easy-to-implement platform for the identification of potent biotherapeutics to address autoimmune pathologies.
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