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Developing novel tetraubiquitin substrates and AlphaLISA technology to provide next generation deubiquitinase assays
This application note presents novel biochemical DUB assays that use TUBEs to bind to tetraubiquitin substrates of at least three natural linkages. These assays, combined with Revvity’s AlphaLISA™ technology, allow for the measurement of protein interactions and large protein complexes in a no-wash, homogeneous assay.
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Detection of human matrix metalloproteinase 9 in complex sample matrices, a comparison of AlphaLISA and colorimetric ELISA immunoassays
This note compares two immunoassay technologies: ELISA (enzyme-linked immunosorbent assay) and AlphaLISA™ (amplified luminescent proximity-based homogeneous assay).
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Tracking inflammasome activity via measuring IL-1β levels with AlphaLISA and HTRF
This application note demonstrates the utility of AlphaLISA™ and HTRF™ for the detection of IL-1β in cell supernatant and serum samples which can be used as a biomarker for inflammasome activity
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Novel high-throughput image cytometry method for T cell immunophenotyping and viability readouts
Application note describing a novel high-throughput image cytometry method for T cell immunophenotyping and viability readouts using the Cellaca PLX image cytometer.
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Characterization of NISTmAb with microfluidic CE-SDS
Application note demonstrating NISTmAb characterization using capillary electrophoresis on the LabChip GXII Touch Protein Characterization System.
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An evaluation of control analysis system performance between the LabChip GXII and SCIEX PA800 Plus system
In this app note mAbs were evaluated using the Protein Clear™ HR assay and an orthogonal method, the SCIEX® PA800 Plus system.
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A quick and direct Candida albicans lysis efficiency comparison using the Cellometer X2 fluorescent cell viability counter.
Application note for Cellometer X2Candida albicans lysis efficiency
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Reliable quantification of Candida albicans by Cellometer X2 cell counter: correlation study with qPCR and ddPCR
Application note for Cellometer X2 correlation study with qPCR and ddPCR
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Guidelines for optimization of a cell-labelling method for DELFIA-TRF cytotoxicity assays.
Discover expert guidelines for optimizing cell labeling in DELFIA TRF cytotoxicity assays. Learn how to enhance assay performance, manage spontaneous release, and achieve accurate results with this comprehensive application note.
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Streamlining the development of bispecific antibodies from expression to quality assessment with Revvity’s biotherapeutic workflow solutions
Expression and characterization of bispecific antibodies with a combination of CHOSOURCE TnT Transposon Technology and LabChip to streamline biotherapeutic workflows
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Characterizing chemokine receptor inhibitors with AlphaLISA SureFire Ultra, Alpha SureFire Ultra Multiplex and LANCE Ultra cAMP assays
This application note demonstrates how the SureFire Ultra and LANCE Ultra cAMP assays can be used for measuring inhibitors to CCR7 and CXCR2 cell surface receptors using a cellular model system where these receptors are overexpressed in CHO cells.
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Simultaneous measurement of chemokine/cytokine release and antibody-dependent cellular cytotoxicity upon binding with rituximab
Simultaneous detection of ADCC activity and release of chemokines and cytokines from activated NK cells is possible via the combined use of DELFIA TRF and AlphaLISA technologies.
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