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Application Note
Developing novel tetraubiquitin substrates and AlphaLISA technology to provide next generation deubiquitinase assays
This application note presents novel biochemical DUB assays that use TUBEs to bind to tetraubiquitin substrates of at least three natural linkages. These assays, combined with Revvity’s AlphaLISA™ technology, allow for the measurement of protein interactions and large protein complexes in a no-wash, homogeneous assay.
Application Note
Detection of human matrix metalloproteinase 9 in complex sample matrices, a comparison of AlphaLISA and colorimetric ELISA immunoassays
This note compares two immunoassay technologies: ELISA (enzyme-linked immunosorbent assay) and AlphaLISA™ (amplified luminescent proximity-based homogeneous assay).
Application Note
A novel method for T-cell immunophenotyping using the Cellometer Spectrum image cytometer.
Application note describing a novel method for T-cell immunophenotyping using the Cellometer Spectrum image cytometer.
Application Note
An alternative image-based technique for phytoplankton cell counts in shellfish aquaculture.
Application note describing an alternative image-based technique for phytoplankton cell counts in shellfish aquaculture using the Cellometer Auto T4 brightfield cell counter.
Application Note
Insect cell counting and size analysis using the Cellometer Auto T4 brightfield cell counter.
Application note descrbing counting and size analysis of insect cells using the Cellometer Auto T4 brightfield cell counter.
Application Note
Cell counting repeatability and consistency using the Cellometer Auto T4 brightfield cell counter.
Cell counting repeatability and consistency using the Cellometer Auto T4 brightfield cell counter.
Application Note
Performing AlphaLISA assays with RPMI-1640
This application note provides alternatives for working with samples in RPMI medium, which contains high levels of biotin
Application Note
Guidelines for optimization of a cell-labelling method for DELFIA-TRF cytotoxicity assays.
Discover expert guidelines for optimizing cell labeling in DELFIA TRF cytotoxicity assays. Learn how to enhance assay performance, manage spontaneous release, and achieve accurate results with this comprehensive application note.
Application Note
Novel high-throughput image cytometry method for T cell immunophenotyping and viability readouts
Application note describing a novel high-throughput image cytometry method for T cell immunophenotyping and viability readouts using the Cellaca PLX image cytometer.
Application Note
Measuring strong to weak binding interactions with AlphaLISA
In this application note, we demonstrate how AlphaLISA can be used to measure three very different types of biomolecular interactions with three very different binding affinities.
Application Note
Functional GPCR Studies Using AlphaScreen cAMP Detection Kit
Read this application note to access detailed data and results on GPCR studies using AlphaScreen cAMP detection kit
Application Note
Comparison of EMT Biomarker Expression in 2D Monolayer and 3D Spheroid Cultures in a Prostate Cancer
This application note shows that AlphaLISA can be used to compare differences in protein expression levels and cellular tolerance for compound treatment between a human prostate cancer cell line grown in monolayers and those same cells grown in 3D spheroids.
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