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Guide
The issue of CHO HCPs during biopharmaceutical production
Detecting and quantifying CHO HCPs with automatable homogeneous immunoassays
Literature - Publication Review
Key signaling pathways in liquid cancer: therapeutic targets, screening tools, and future developments
This review highlights some of these signaling pathways and demonstrates how assays based on HTRF and AlphaLISA technologies can be used in the screening and development of new therapies for leukemia and lymphoma treatment.
Application Note
Developing novel tetraubiquitin substrates and AlphaLISA technology to provide next generation deubiquitinase assays
This application note presents novel biochemical DUB assays that use TUBEs to bind to tetraubiquitin substrates of at least three natural linkages. These assays, combined with Revvity’s AlphaLISA™ technology, allow for the measurement of protein interactions and large protein complexes in a no-wash, homogeneous assay.
Application Note
Streamline the HEK 293 HCP impurity quantification workflow with new, no-wash AlphaLISA HEK 293 Host Cell Protein Kit
Read our new HEK HCP application note to learn about HEK 293 HCP impurity quantification using AlphaLISA technology (link not available yet)
Application Note
Advantages of using our automatable and no-wash HTRF and AlphaLISA kits for CHO HCP detection
Read this application note to learn about CHO HCP impurity quantification using AlphaLISA and HTRF technologies
Application Note
Measuring strong to weak binding interactions with AlphaLISA
In this application note, we demonstrate how AlphaLISA can be used to measure three very different types of biomolecular interactions with three very different binding affinities.
Application Note
cAMP assay provides flexibility and stable pharmacology
AlphaScreen™ technology provides a quantitative detection of molecules in a homogeneous, no-wash format and can be applied to GPCR research.
Technical Note
Biotin-free AlphaLISA assays for the detection of cytokines in PBMC supernatants
In this technical note, AlphaLISA biotin-free kits were used to detect and quantify the cytokines interleukin 2 (IL-2), tumor necrosis factor alpha (TNFα), interferon gamma (IFN-γ), and interleukin 6 (IL-6).
Application Note
Functional GPCR Studies Using AlphaScreen cAMP Detection Kit
Read this application note to access detailed data and results on GPCR studies using AlphaScreen cAMP detection kit
Application Note
Avoiding biotin interference in AlphaLISA assays
This application note demonstrates the value of using AlphaLISA biotin-free kits to reduce the effects of biotin interference in sample and standard preparations.
Whitepaper
Alpha technologies for antibody detection and characterization
Alpha technology is homogeneous and non-radiometric with distinct features that makes it enabling in comparison to other proximity assays.
Application Note
Comparison of EMT Biomarker Expression in 2D Monolayer and 3D Spheroid Cultures in a Prostate Cancer
This application note shows that AlphaLISA can be used to compare differences in protein expression levels and cellular tolerance for compound treatment between a human prostate cancer cell line grown in monolayers and those same cells grown in 3D spheroids.
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