IVISense 680 NHS Fluorescent Dye (1 mg) (VivoTag)

IVISense 680 NHS fluorescent dye, at a wavelength 680 nm, is a amine-reactive near-infrared fluorochrome for coupling via an NHS ester linkage to peptides, small molecules, proteins, antibodies, nanoparticles or other macromolecules. Hydrolytically stable and low self quenching for higher loading applications. Unit size 1 mg.

IVISense™ 680 fluorescent dyes are ideal for molecular labeling for use in preclinical in vivo imaging applications and in vitro corroboration assessments by microscopy and flow cytometry. IVISense 680 dyes are well suited for the labeling and imaging of proteins, antibodies, and small molecules in a wide range of research areas. The 680 nm fluorescence wavelength is ideal for biological imaging applications using in vivo fluorescence systems and provides sufficient signal in most advanced microscopes, high content imaging and flow cytometry.

• Uniquely optimized for the generation of in vivo imaging probes with low non-specific accumulation at tumor sites and inflammatory sites.
• Suboptimal but sufficient excitation and emission with light sources and filters in all commonly used microscopes, flow cytometers, and plate readers.
• Suitable for in vivo multiplexing with 750 or 800 nm Revvity probes or other labeled proteins/Abs. Expanded in vivo multiplexing with IVIS spectral unmixing with 645/750/800 nm probes or antibodies.
• Requires maintaining mice on alfalfa-free diet to remove spectral overlap with diet autofluorescence.
• Recommended dye:protein ratio of 1-3.