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AlphaLISA Human IL-6 Detection Kit, 500 Assay Points
AlphaLISA Human IL-6 Detection Kit, 500 Assay Points
AlphaLISA Sandwich Anti-analyte Conjugated Acceptor Bead
The AlphaLISA™ Human Interleukin-6 (IL6) Detection Kit is designed for detection and quantitation of human IL-6 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
| Feature | Specification |
|---|---|
| Application | Protein Quantification |
| Dynamic Range | 1.3 - 30,000 pg/mL |
| Limit of Detection | 1.3 pg/mL |
| Sample Volume | 5 µL |
The AlphaLISA™ Human Interleukin-6 (IL6) Detection Kit is designed for detection and quantitation of human IL-6 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
Product Variants
Unit Size: 500 assay points
Part #:
AL223C
List Price
USD 2,463.76
Your online price:
Unit Size: 5,000 assay points
Part #:
AL223F
List Price
USD 16,428.50
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For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).
AlphaLISA Human IL-6 Detection Kit, 500 Assay Points
AlphaLISA Sandwich Anti-analyte Conjugated Acceptor Bead
AlphaLISA Human IL-6 Detection Kit, 500 Assay Points
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Product information
Overview
Formats:
- Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
- Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
Features:
- No-wash steps, no separation steps
- ELISA alternative technology
- Sensitive detection
- Broad sample compatibility
- Small sample volume
- Results in less than 3 hours
- Half time of an ELISA assay
Interleukin 6 (IL6) is a ~22 kDa pleiotropic cytokine that acts not only on immune system, but also affects many physiological events in various organs. IL6 exerts pro- or anti-inflammatory effects, depending on target cell analyzed and in vivo environmental circumstances. IL6 is a differentiation and proliferation factor B and T cells, and acts as a migration factor on monocytic cells. It is major activator of acute-phase protein expression in liver, a hematopoietic factor, and acts as a survival factor on neuronal cells. IL6 signals through binding to gp130/ IL-6R receptor complex, leading to activation of JAK/STAT, MAPK and PI3K cascades.
AlphaLISA technology allows detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to Streptavidin-coated Donor beads while anor anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In presence of analyte, beads come into close proximity. excitation of Donor beads causes release of singlet oxygen molecules that triggers a cascade of energy transfer in Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
Specifications
| Application |
Protein Quantification
|
|---|---|
| Automation Compatible |
Yes
|
| Brand |
AlphaLISA
|
| Detection Modality |
Alpha
|
| Dynamic Range |
1.3 - 30,000 pg/mL
|
| Limit of Detection |
1.3 pg/mL
|
| Product Group |
Kit
|
| Sample Volume |
5 µL
|
| Shipping Conditions |
Shipped in Blue Ice
|
| Target |
IL6
|
| Target Class |
Cytokines
|
| Target Species |
Human
|
| Technology |
Alpha
|
| Therapeutic Area |
Inflammation
|
| Unit Size |
500 assay points
|
Video gallery
AlphaLISA Human IL-6 Detection Kit, 500 Assay Points
AlphaLISA Human IL-6 Detection Kit, 500 Assay Points
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Resources
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Application Note
Comparison of EMT Biomarker Expression in 2D Monolayer and 3D Spheroid Cultures in a Prostate Cancer
AlphaLISA and LANCE (TR-FRET) biomarker assays can be used to measure ECM-associated protein modulation caused by human...
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