16S rRNA Library Prep Solutions

Revvity now brings flexibility to your microbial community profiling projects with three 16S library prep kits, each covering different regions in the 16S rRNA gene.

• Robust, fast and easy library prep protocol
• Low input – Only 1 ng of input required
• Options now available with the CosmosID-Hub® solution to simplify analysis
• Multiplex up to 384 libraries
• Automation protocols available for the Sciclone™ NGS and NGSx workstations

These 16S rRNA sequencing kits are available alone or bundled with the CosmosID-Hub^® analysis solution, delivering user-friendly access to version-controlled 16S pipelines.

Revvity 16S rRNA Analysis - Online Demonstration

Now you can schedule a one-on-one 30-minute online demonstration will cover everything you need to know about the CosmosID-Hub® which is now available with Revvity’s NEXTFLEX™ 16S rRNA Amplicon-seq Kits. You will be able to see the analysis demonstrated and our experts will be able to answer any questions that you may have. Please choose a timeframe that suits you best and we will provide you a Microsoft Teams personal link to join when the time comes. We look forward to speaking with you.

NEXTFLEX™ 16S V4 Amplicon-Seq kit 2.0

Researchers often choose the V4 region because of the availability of reference databases (e.g., SILVA). it provides sufficient diversity to allow discrimination of genus and sometimes bacterial species while avoiding excessive noise from hyper-variable regions.

Additionally, it is relatively short, making it an effective choice for Illumina® sequencing platforms.

NEXTFLEX 16S V3-V4 Amplicon-Seq kit

This kit offers comparable benefits. Widely used and well established, V3-V4 data has contributed to community diversity metrics such as UniFrac and Faith’s phylogenetic diversity.

NEXTFLEX 16S V1-V3 Amplicon-Seq kit

The V1-V3 region provides higher taxonomic precision for specific microbial taxa. Researchers planning to use this region must weigh the trade-offs between taxonomic precision and comprehensive community profiling.

Learn more about these cutting-edge 16S library prep solutions below and revolutionize your research.